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2D cross-sections of 3D two-photon microscopy images of different axon terminals with filopodia

Journal: BMC Bioinformatics

Article Title: Semi-automatic geometrical reconstruction and analysis of filopodia dynamics in 4D two-photon microscopy images

doi: 10.1186/s12859-026-06385-4

Figure Lengend Snippet: 2D cross-sections of 3D two-photon microscopy images of different axon terminals with filopodia

Article Snippet: 3D tracing of filopodia can be achieved using one of the commercial microscopy image analysis tools, such as Imaris .

Techniques: Microscopy

3D images of example axon terminals showing filopodia, visualized using volume rendering. a Two-photon microscopy images of two axon terminals shown in x-y orientation. b The axial resolution in z direction (here the horizontal direction) is poorer (0.1 × 0.1 × 05 μm 3 )

Journal: BMC Bioinformatics

Article Title: Semi-automatic geometrical reconstruction and analysis of filopodia dynamics in 4D two-photon microscopy images

doi: 10.1186/s12859-026-06385-4

Figure Lengend Snippet: 3D images of example axon terminals showing filopodia, visualized using volume rendering. a Two-photon microscopy images of two axon terminals shown in x-y orientation. b The axial resolution in z direction (here the horizontal direction) is poorer (0.1 × 0.1 × 05 μm 3 )

Article Snippet: 3D tracing of filopodia can be achieved using one of the commercial microscopy image analysis tools, such as Imaris .

Techniques: Microscopy